Do bacteria can form biofilms on glass?

Do bacteria form biofilms on microscope slides? We would like to count number of bacteria attached to the artificial surfaces formed on microscope slides and to use conventional microscope slides...

31 December 2018 2,479 14 View

What species of bacteria in human oral cavity are cultivable?

Can anyone suggest the short list or just to mention bacterial species (not only genera) (up to 10-15 species) from human oral cavity (particularly gram-negatives, causing periodontal diseases)...

05 June 2017 1,806 3 View

PCR primers for detection of Fusobacterium necrophorum and Bacteroides melaninogenicus ?

09 October 2016 4,145 3 View

Selective media for isolation of resistant bacteria from soil?

We would like to isolate resistant bacteria from soil however, only certain species has established their epidemiological cut-off values or clinical breakpoints. Any method for initial isolation...

08 September 2015 935 4 View

Microbiota in soil: best method for isolation?

Anyone can suggest method for concentration and isolation (as much as possible) bacteria from soil? I guess it is impossible to use filters as are used for water filtration (as soil suspension is...

08 September 2015 1,725 0 View

What are the best primers for 16S rRNA sequencing for identification of soil bacteria isolates?

08 September 2015 2,948 25 View

Is there a dye which changes colour in contact with bacteria?

Could anybody suggest dye that changes colour in contact with bacteria or pus?

02 March 2015 1,209 14 View

How can I identify the staphylococcus species using sequencing?

I have extracted DNA from different species of staphylococci. I want to make a PCR and then to sequence the obtained product for staphylococcus species determination using BLAST search. However I...

11 December 2013 6,297 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View