Do bacteria can form biofilms on glass?

Do bacteria form biofilms on microscope slides? We would like to count number of bacteria attached to the artificial surfaces formed on microscope slides and to use conventional microscope slides...

31 December 2018 2,479 14 View

What species of bacteria in human oral cavity are cultivable?

Can anyone suggest the short list or just to mention bacterial species (not only genera) (up to 10-15 species) from human oral cavity (particularly gram-negatives, causing periodontal diseases)...

05 June 2017 1,806 3 View

PCR primers for detection of Fusobacterium necrophorum and Bacteroides melaninogenicus ?

09 October 2016 4,145 3 View

Selective media for isolation of resistant bacteria from soil?

We would like to isolate resistant bacteria from soil however, only certain species has established their epidemiological cut-off values or clinical breakpoints. Any method for initial isolation...

08 September 2015 935 4 View

What are the best primers for 16S rRNA sequencing for identification of soil bacteria isolates?

08 September 2015 2,948 25 View

Is there a dye which changes colour in contact with bacteria?

Could anybody suggest dye that changes colour in contact with bacteria or pus?

02 March 2015 1,209 14 View

Can anybody suggest species-specific primers for Chlamydophila psittaci and Mycobacterium avium?

01 February 2015 9,440 5 View

How can I identify the staphylococcus species using sequencing?

I have extracted DNA from different species of staphylococci. I want to make a PCR and then to sequence the obtained product for staphylococcus species determination using BLAST search. However I...

11 December 2013 6,297 4 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View