Anyone can suggest method for concentration and isolation (as much as possible) bacteria from soil? I guess it is impossible to use filters as are used for water filtration (as soil suspension is too dirty)? Any special methods?
Do bacteria form biofilms on microscope slides? We would like to count number of bacteria attached to the artificial surfaces formed on microscope slides and to use conventional microscope slides...
31 December 2018 2,602 14 View
Can anyone suggest the short list or just to mention bacterial species (not only genera) (up to 10-15 species) from human oral cavity (particularly gram-negatives, causing periodontal diseases)...
05 June 2017 2,006 3 View
Can anybody suggest working primers for CONVENTIONAL PCR (not a real-time PCR) to detect Fusobacterium necrophorum and Bacteroides melaninogenicus? Data on PCR conditions and product size (in bp)...
09 October 2016 4,269 3 View
We would like to isolate resistant bacteria from soil however, only certain species has established their epidemiological cut-off values or clinical breakpoints. Any method for initial isolation...
08 September 2015 1,058 4 View
I would like to identify (by 16SrRNR sequencing and blasting) bacteria (pure cultures) prevalent in a soil. Which primer pair or sets of primer pairs would be the most useful for that reason?
08 September 2015 3,084 25 View
Could anybody suggest dye that changes colour in contact with bacteria or pus?
02 March 2015 1,487 14 View
Can anybody suggest species-specific primers for conventional PCR (one reaction) for detection of Chlamydophila psittaci and Mycobacterium avium in birds?
01 February 2015 9,557 5 View
I have extracted DNA from different species of staphylococci. I want to make a PCR and then to sequence the obtained product for staphylococcus species determination using BLAST search. However I...
11 December 2013 6,442 4 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?
01 August 2024 1,180 2 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View