Can I use Sephadex G-200 to separate a complex of 2 Megadalton from a 100,000 protein?

I have 1 ml of 0.4 mg protein which I want to separate. Mainly I want the complex which should come in the void volume. I used a 40 ml bed volume manually packed column (0.75 cm diameter), but...

05 June 2018 4,767 3 View

Does anyone know why the retroviral shRNAs do not work at a later time point?

We are using retroviral shRNAs (both tagged and untagged) and made glycerol stocks after getting them from Origin. Have used them a number of times with proper downregulation profile. But now they...

03 April 2018 4,255 3 View

Can anyone suggest transfection methods for big inserts in neuroblstoma or glioblastoma cell lines?

Does anybody know why the transfection of alpha-fodrin (250 kda) is so less efficient in cell lines like glioblastoma U251 and neuroblastoma IMR 32? It works well in HEK293. I have tried both...

02 March 2017 2,660 4 View

Is there any cell lysis buffer which will extract only cytosolic proteins, leaving other organelles?

I want to avoid centrosomal proteins.

10 November 2013 4,662 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View