Suparna Sengupta

5 Questions 14 Answers 0 Followers

Questions related from Suparna Sengupta

Can I use Sephadex G-200 to separate a complex of 2 Megadalton from a 100,000 protein?

I have 1 ml of 0.4 mg protein which I want to separate. Mainly I want the complex which should come in the void volume. I used a 40 ml bed volume manually packed column (0.75 cm diameter), but...

06 June 2018 4,859 3 View

Does anyone know why the retroviral shRNAs do not work at a later time point?

We are using retroviral shRNAs (both tagged and untagged) and made glycerol stocks after getting them from Origin. Have used them a number of times with proper downregulation profile. But now they...

04 April 2018 4,330 3 View

Can anyone suggest transfection methods for big inserts in neuroblstoma or glioblastoma cell lines?

Does anybody know why the transfection of alpha-fodrin (250 kda) is so less efficient in cell lines like glioblastoma U251 and neuroblastoma IMR 32? It works well in HEK293. I have tried both...

03 March 2017 2,736 4 View

Can anybody suggest some adherent cancer cell lines with high intrinsic c-myc levels?

I want to study the role of c-myc in some downstream processes. For that I need to start with cells intrisincally overexpressing c-myc. Can anybody suggest some adherent lines?

12 December 2014 7,643 6 View

Is there any cell lysis buffer which will extract only cytosolic proteins, leaving other organelles?

I want to avoid centrosomal proteins.

11 November 2013 4,739 5 View