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Questions related from Suparna Sengupta
I have 1 ml of 0.4 mg protein which I want to separate. Mainly I want the complex which should come in the void volume. I used a 40 ml bed volume manually packed column (0.75 cm diameter), but...
06 June 2018 4,865 3 View
We are using retroviral shRNAs (both tagged and untagged) and made glycerol stocks after getting them from Origin. Have used them a number of times with proper downregulation profile. But now they...
04 April 2018 4,336 3 View
Does anybody know why the transfection of alpha-fodrin (250 kda) is so less efficient in cell lines like glioblastoma U251 and neuroblastoma IMR 32? It works well in HEK293. I have tried both...
03 March 2017 2,740 4 View
I want to study the role of c-myc in some downstream processes. For that I need to start with cells intrisincally overexpressing c-myc. Can anybody suggest some adherent lines?
12 December 2014 7,650 6 View
I want to avoid centrosomal proteins.
11 November 2013 4,745 5 View