Open source glycopeptide finding software using windows platform?

Hello, I was working with a human plasma sample which was supposed to be rich in glycoprotein. Now after processing the sample and running them on Orbitrap fusion lumos I need to search...

07 August 2019 4,859 4 View

How to separate low mw proteins in solution?

Hello, I need help on something. I was having trouble in doing an experiment. Do you guys know any method or established protocol to separate protein by mw using simple spin column? Suppose,...

06 July 2019 1,498 2 View

DNA Extraction from Monilinia fructicola (Fungi)?

I am doing DNA extraction using CTAB method from different fungal strains including monilinia spp. isolated from peach. My DNA concentration is very low. Is there any suggestion to improve the...

21 January 2019 8,673 3 View

Best Linear Unbiased Predictions (BLUP)??

I need an urgent help from the researchgate community. Can anybody help me to write codes for BLUP model in R (any BLUP package) for the attached data? First column of the .csv file indicate...

08 September 2018 391 2 View

What i have done Wrong? Primers are degradded or cDNA of miRNA?

I was running RT-PCR for miRNA expression analysis from mice tissue last week but i get nothing in there.. I write detail of experiments. Guide me if i have made a mistake. 1. Total RNA Isolation...

30 August 2018 3,492 4 View

Is there some theory which explains why the women, who as children saw their fathers abusing their mothers, accept spousal violence as adults?

There is some empirical evidence to suggest that the women who saw their fathers physically abusing their mothers in their childhood are more likely to justify spousal violence as adults compared...

07 August 2018 182 7 View

I am working on insect immunity and my qRT-PCR, western blot and iTRAQ data do not have concordance?

I repeated all qRT-PCR and western blot few times but got the same results every time. I would be glade if someone explain why there is difference between these three data for the same gene?? Thanks

31 December 2017 8,245 4 View

What methods are available for removing SDS from protein for Top Down Proteomics analysis?

02 October 2017 6,400 7 View

How to Store Bifidobacteria Befidum?

I received Bifidobacteria Befidum powder from a company. I have to start culturing and start using accordingly. I want to store part of it for future use. Which option is best one? 1. Either store...

04 March 2017 708 6 View

1. Which genotyping approach is to be adapted (WGS, GBS or Chip) under existing situation of poor and unreliable records?

Can we use 770 K Illumina bovine chip or we should go for whole genome sequencing and develop a new chip or we should use GBS approach? Which approach could be most economical? If we use 770 K...

01 February 2017 4,882 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View