How to separate low mw proteins in solution?

Hello, I need help on something. I was having trouble in doing an experiment. Do you guys know any method or established protocol to separate protein by mw using simple spin column? Suppose,...

06 July 2019 1,498 2 View

DNA Extraction from Monilinia fructicola (Fungi)?

I am doing DNA extraction using CTAB method from different fungal strains including monilinia spp. isolated from peach. My DNA concentration is very low. Is there any suggestion to improve the...

21 January 2019 8,673 3 View

Best Linear Unbiased Predictions (BLUP)??

I need an urgent help from the researchgate community. Can anybody help me to write codes for BLUP model in R (any BLUP package) for the attached data? First column of the .csv file indicate...

08 September 2018 391 2 View

What i have done Wrong? Primers are degradded or cDNA of miRNA?

I was running RT-PCR for miRNA expression analysis from mice tissue last week but i get nothing in there.. I write detail of experiments. Guide me if i have made a mistake. 1. Total RNA Isolation...

30 August 2018 3,492 4 View

Is there some theory which explains why the women, who as children saw their fathers abusing their mothers, accept spousal violence as adults?

There is some empirical evidence to suggest that the women who saw their fathers physically abusing their mothers in their childhood are more likely to justify spousal violence as adults compared...

07 August 2018 182 7 View

I am working on insect immunity and my qRT-PCR, western blot and iTRAQ data do not have concordance?

I repeated all qRT-PCR and western blot few times but got the same results every time. I would be glade if someone explain why there is difference between these three data for the same gene?? Thanks

31 December 2017 8,245 4 View

What methods are available for removing SDS from protein for Top Down Proteomics analysis?

02 October 2017 6,400 7 View

How to Store Bifidobacteria Befidum?

I received Bifidobacteria Befidum powder from a company. I have to start culturing and start using accordingly. I want to store part of it for future use. Which option is best one? 1. Either store...

04 March 2017 708 6 View

1. Which genotyping approach is to be adapted (WGS, GBS or Chip) under existing situation of poor and unreliable records?

Can we use 770 K Illumina bovine chip or we should go for whole genome sequencing and develop a new chip or we should use GBS approach? Which approach could be most economical? If we use 770 K...

01 February 2017 4,882 3 View

Can someone guide me how to construct layers in graphene using wien2k code?

plz guide me, how to construct layers in graphene using wien2k code????

20 December 2015 821 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View