I already tried with double thymidine block and nocodazole treatment but i did not get synchronized samples. Suggest me suitable protocol for the same.
Thank u Sabine but I already tried with double thymidine block and nocodazole. I used different time points for double thymidine block for MCF7 but after FACS I came to know that cells were not synchronized. Can you please help me for MCF7 by using thymidine.