How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

How to check solubility of PHA?

I have extracted mcl PHA from bacteria. But I am facing problem in dissolving the extracted PHA. It is not dissolving in chloroform and other solvents like acetone, dichloroethane, diethylether...

29 October 2020 7,794 2 View

Best metallurgy to prevent hot sulfidation?

Please note that I am an inspection engineer and would like your views/ opinion on the following - We have experienced severe metal wastage and tube failure in a heat exchanger with SS304 tubes....

26 March 2020 9,877 6 View

How to dissolve Ethylene Vinyl Acetate?

I need to get rid of the ethylene vinyl acetate paste stuck onto a glass surface. What would be the best solvent to get rid of it?

09 August 2019 9,016 6 View

acidic and basic buffers?

list would be good

27 February 2019 8,462 3 View

I have to add IL-2 signal peptide in front of gene of interest in pcdna 3.1 vector to export it out of the cells after transfection. How to do that?

My Questions are: 1. Should the gene of interest be directly after the IL-2 sequence and if that is the case, should it have its own start codon (ATG)? 2, if it has to have its own ATG, would that...

02 May 2016 5,593 3 View

How will you account for the following changes in 19^ F- NMR spectrum of SF4?

11 December 2014 1,084 7 View

How will Cis and Trans forms of square planar complex Pt(NH3)2(NO3)2 react with oxalic acid?

Square planar Pt(NH3)2(NO3)2 exists both in the Cis and Trans forms. Will the two isomers give same or different products with oxalic acid ? Please write equation/s in your answers. Thank you.

10 December 2014 1,602 2 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

How to measure the hardness for PLA & PHAs polymers?

Hello, I am a bit confused on whether to follow ASTM D785 (Standard Test Method for Rockwell Hardness of Plastics and Electrical Insulating Materials) or ASTM D2240 (Standard Test Method for...

21 January 2021 7,487 4 View

How to prepare solid polyhydroxyalkanoate (PHA) polymers for in solution Nile Blue A staining analysis?

Hi all, I am trying to make a standard curve for Nile Blue A fluorescence using Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) as the standard and a plate reader to measure fluorescence. My issue...

02 January 2021 3,027 2 View

During my work in the laboratory?

During my work in the laboratory, I noticed that fructose, starch and dextrin did not give positive results with their statements such as iodine and sulwanof when prepared 3 to 5 days ago. Why?

25 December 2020 8,390 5 View

What's a cost effective way to make starch based nanocomposite hydrogels in large scale?

starch based nanocomposite hydrogels

13 December 2020 431 4 View

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

08 December 2020 2,087 1 View

Zeta Potential measurement of starch solution?

I want to measure the charge on the particles in the solutions using zetasizer, but the experiment needs R.I and absorbance co-efficient to be conducted, but i cant find the stated valued for the...

08 November 2020 3,691 9 View

How to check solubility of PHA?

I have extracted mcl PHA from bacteria. But I am facing problem in dissolving the extracted PHA. It is not dissolving in chloroform and other solvents like acetone, dichloroethane, diethylether...

29 October 2020 7,794 2 View

Does anybody have experiences with FFF separation of PHB particles?

I am trying to separate suspension of PHB particles, stabilized with PVA. The DLS results show two populations with peak maxima about 150 nm and 380 nm. The Zeta potential was -3 mV (pH 7) across...

21 October 2020 6,551 1 View