In order to make a good standarization, one should have the same number of bacteria from different conditions tested to examine the expression of the reference genes? is there any other way I might not taking notice of?
I'm thinking about to do a direct counting with a Petroff Hausser chamber , and when the bacteria reach the mid log phase do dilutions to have the same number in both conditions, extract RNA , convert to cDNA and carry the qPCR.
I would like to know some recommendations to do this task and to appeal to your experience.
Hello Gregory,
In my research I evaluate five candidate reference genes as well as different quantification methods for qPCR.
Have a look.
Hope it helps.
http://onlinelibrary.wiley.com/doi/10.1111/jam.12740/pdf
- Badges
- Science topic