I've been obtaining mixed results regarding to duplication time in bacterial kinetics of some strain I'm studying.
Duplication times vary from 54 to 64 hrs
How much variation should be allowed?
Dear Gregory,
The following is a Table lists variation time for some bacteria:
Bacterium Medium Generation Time (minutes)
Escherichia coli Glucose-salts 17
Bacillus megaterium Sucrose-salts 25
Streptococcus lactis Milk 26
Streptococcus lactis Lactose broth 48
Staphylococcus aureus Heart infusion broth 27-30
Lactobacillus acidophilus Milk 66-87
Rhizobium japonicum Mannitol-salts-yeast extract 344-461
Mycobacterium tuberculosis Synthetic 792-932
Treponema pallidum Rabbit testes 1980
Hoping this will be helpful,
Rafik
Dear Gregory,
The following is a Table lists variation time for some bacteria:
Bacterium Medium Generation Time (minutes)
Escherichia coli Glucose-salts 17
Bacillus megaterium Sucrose-salts 25
Streptococcus lactis Milk 26
Streptococcus lactis Lactose broth 48
Staphylococcus aureus Heart infusion broth 27-30
Lactobacillus acidophilus Milk 66-87
Rhizobium japonicum Mannitol-salts-yeast extract 344-461
Mycobacterium tuberculosis Synthetic 792-932
Treponema pallidum Rabbit testes 1980
Hoping this will be helpful,
Rafik
Clearly you are working with slow growing bacteria.
Has the variation been observed between parallel cultures of the same isolate, in the same medium, at the same incubation temperature, pO2, pC02 and so on?
How do you monitor growth? Do you estimate doubing time in the log phase?
Yes I have.
same culture conditions, I estimate doubling time in log phase, my countings are done using Petroff Hausser counting chamber.
If you wish, send me an excel sheet with all the raw experimental points of one of the experiments that worry you, An ANOVA analysis might clarify the situation.
My mail: [email protected]
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