Is there a graphical simulation program for proteins, where you can attach reporter molecules to specific aminoacids or motifs?

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Technical triplicates input in REST2009?

Greetings to all: I would like to know what is the right manner to input: -the ct values of technical triplicates and biological triplicates of my control and sample values. -will REST2009...

08 September 2016 2,899 0 View

Are these reference genes (relative quantification qPCR) suitable according to results?

Greetings to you all: -I only got to test 4 ref. genes -In spite of that, I obtain in geNorm M values lower than 0.5 and the best couple got a 0.217 M value. -Then, I chosed 3 ref. genes since...

07 August 2016 5,599 1 View

Troubles regarding plate design for qPCR experiment...help?

hello everyone, i hope you can help me about this troublesome quest: -I will study 2 conditions with 3 biological replicates (3 samples in control vs 3 samples in treatment) (6 samples in...

07 August 2016 8,361 4 View

What would be the logical protocol for reference gene search in bacteria?

First, I am thinking about extracting RNA from the same number of bacteria from 2 differents conditions. Secondly, there would be a quantification and normalization step of RNA ammounts? In...

31 December 2015 1,383 2 View

Duplication time in bacteria: How much variation should be allowed?

I've been obtaining mixed results regarding to duplication time in bacterial kinetics of some strain I'm studying. Duplication times vary from 54 to 64 hrs How much variation should be allowed?

10 November 2015 4,103 5 View

Is an exponential regression coefficient of 0.96 enough for Bacterial Growth?

During the phase of exponential growth in my bacteria, I have managed to stablish a exponential regression coefficient of 0.96. Is this enough to claim that this is in fact and exponential...

09 October 2015 5,550 3 View

Can one "correct" dilutions prepared for qPCR standard curve?

Im having trouble getting the right efficency for my primers doing the standart procedure of serial dilutions. But I soon as I correct the dilutions using a quantifyer like Qubit reagent for...

04 May 2015 1,862 6 View

Same ammount of bacteria OR same ammount of RNA for Reference Genes anaIysis , searching and finding?

I have this question that I havent been abIe to answer by Iooking at the Iiterature, everyone seems to assume something Im obviousIy missing, which is: when you are doing an assay of Reference...

01 February 2015 8,368 1 View

Is it possibLe to perform RT-PCR with different Hybridization Temps?

Is it possible to perform RT PCR assays were targets have different TH? Especially reference genes, doing some reference genes at one reaction with some targets and the rest with the other sampIes?

31 December 2014 4,037 2 View

What is the most important parameter for qPCR primer design?

HeIIo dear aII: Im aIready famiIiriazed with parameters needed for qpcr primer design, so I have made the design manuaIIy with PRIMER 3 and OIigoAnaIizer and with private software Primer express...

11 December 2014 9,110 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View