15 Questions 33 Answers 0 Followers
Questions related from Gregory Guerra Bieberach
greetings to you all, I require to see if there actually exist some simulation programs for proteins with includes the adition of reporters like GFP, FRET, ANTIBODIES, APTAMERS, etc thank you...
10 October 2016 662 2 View
Greetings to all: I would like to know what is the right manner to input: -the ct values of technical triplicates and biological triplicates of my control and sample values. -will REST2009...
09 September 2016 2,866 0 View
hello everyone, i hope you can help me about this troublesome quest: -I will study 2 conditions with 3 biological replicates (3 samples in control vs 3 samples in treatment) (6 samples in...
08 August 2016 8,330 4 View
Greetings to you all: -I only got to test 4 ref. genes -In spite of that, I obtain in geNorm M values lower than 0.5 and the best couple got a 0.217 M value. -Then, I chosed 3 ref. genes since...
08 August 2016 5,566 1 View
First, I am thinking about extracting RNA from the same number of bacteria from 2 differents conditions. Secondly, there would be a quantification and normalization step of RNA ammounts? In...
01 January 2016 1,351 2 View
I've been obtaining mixed results regarding to duplication time in bacterial kinetics of some strain I'm studying. Duplication times vary from 54 to 64 hrs How much variation should be allowed?
11 November 2015 4,062 5 View
During the phase of exponential growth in my bacteria, I have managed to stablish a exponential regression coefficient of 0.96. Is this enough to claim that this is in fact and exponential...
10 October 2015 5,527 3 View
Im having trouble getting the right efficency for my primers doing the standart procedure of serial dilutions. But I soon as I correct the dilutions using a quantifyer like Qubit reagent for...
05 May 2015 1,829 6 View
I have this question that I havent been abIe to answer by Iooking at the Iiterature, everyone seems to assume something Im obviousIy missing, which is: when you are doing an assay of Reference...
02 February 2015 8,332 1 View
Is it possible to perform RT PCR assays were targets have different TH? Especially reference genes, doing some reference genes at one reaction with some targets and the rest with the other sampIes?
01 January 2015 4,005 2 View
HeIIo dear aII: Im aIready famiIiriazed with parameters needed for qpcr primer design, so I have made the design manuaIIy with PRIMER 3 and OIigoAnaIizer and with private software Primer express...
12 December 2014 9,078 4 View
I have tried aII combinations of primers and Temp. for genomic DNA standarts, but I havent got a good efficency, even tho the primers have been reported having a very good efficency using genomic...
12 December 2014 4,042 1 View
Does anyone have any certainty about this?
12 December 2014 6,894 4 View
Is a 3´ end heterodimer of Delta G: -1.6 kcal/mole with 2 base pairs of bridge going to affect the real-time PCR primer efficiency? I would like to know of someone who has used primers with these...
11 November 2014 6,091 4 View
In order to make a good standarization, one should have the same number of bacteria from different conditions tested to examine the expression of the reference genes? is there any other way I...
11 November 2014 1,035 1 View
