Can anybody provide protocol for the lipase inhibition assay using olive oil ?

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Arizaldo Castro

Here are some articles:

Article The modulation of pancreatic lipase activity by alginates

http://www.tandfonline.com/doi/full/10.3109/14756366.2013.871007

Farheen Naz

Thanks for the reply.

Actually i have to see activity against some drugs..

Using olive oil substrate because no other substrate available in my lab.

Ineed the protocol for that.

Adeyemi Oluwadare Ayodeji

Dear Farheen Naz,

Can you be clearer on the question.

Are you studying the inhibitory Effects of the "molecules" on the activity of Lipase from A. niger while using Olive oil as the substrate?

Or you are studying the inhibitory activity of the enzyme on the said molecules?

These are two different studies. Please be clearer on this.