Farheen Naz

12 Questions 13 Answers 0 Followers

Questions related from Farheen Naz

Transfection protocol to transfect A549 CELLS ?

Anyone who can help me in transfection of A549 cells. I have done several times but it doesn't work properly. Thank you so much.

17 July 2023 10,047 5 View

Protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE)?

Hi everyone Can anyone will describe the best protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). I have to do in cardiac cells(primary and...

23 June 2023 821 0 View

Best protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE)?

Hi everyone Can anyone will describe the best protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). I have to do in cardiac cells. Can anyone...

19 June 2023 4,981 0 View

What are different markers to check Multivesicular Bodies (MVBs) for immunofluorescence?

Hello Everyone I have overexpressed one gene in cardiac cells. I notice so MVBs but to confirm that those are MVBs, please can anyone suggest me different markers which I can used to do further my...

06 June 2023 4,997 3 View

What are different markers to check Multivesicular Bodies (MVBs) for immunofluorescence ?

Hello Everyone I have overexpressed one gene in cardiac cells. I notice so MVBs but to confirm that those are MVBs, please can anyone suggest me different markers which I can used to do further...

05 June 2023 6,336 0 View

What is the reason why whole Multivesicular Bodies (MVBs) in cardiac cell are coming out of the cell?

I have overexpressed one gene, and I noticed that whole MVBs are releasing out, instead of forming the bud with the plasma membrane and releasing the stored material. Could someone suggest to me...

03 June 2023 281 0 View

How to wave off fees for publishing in PLOS ONE ?

Please can any body guide my how to apply for fee reduction for publishing my research paper in journal PLOS ONE?

31 December 2021 1,041 6 View

Do there is always an intrinsic uncertainity for quenching data . How can we ensure that our data is correct ?

11 April 2021 5,661 0 View

If the affinity of receptor ligand is high , so is it neccessary it should have high inhibition property?

I did an experiments in which affinity constant of one ligand receptor complex is low as compared to other complexes(other ligands). But it is having high inhibition activity as compared to other...

07 November 2019 8,481 1 View

Can anybody provide protocol for the lipase inhibition assay using olive oil ?

I am using lipase of aspergillus niger for lipase inhibiton assay against some molecules. using substrate olive oil. Kindly if anybody have protocol? Thanks

13 February 2018 4,164 3 View

After dialysis of lipase , how to prevent scattering and aggregation for uv absorption spectra.Kindly suggest what to do ?

I purchased lipase. It is not soluble in water. After doing dialysis, scattering and aggregation has been noticed in UV spectra . What should I do to prevent the above same?

31 October 2017 179 2 View

So how can I calculate binding affinity in this case?

During binding studies the fluorescence measurement of proteins on adding ligand is not showing one pattern.it is firstly decreasing and then start increasing with increase in wavelegnth.

23 March 2017 8,034 4 View