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Questions related from Farheen Naz
Anyone who can help me in transfection of A549 cells. I have done several times but it doesn't work properly. Thank you so much.
17 July 2023 9,983 5 View
Hi everyone Can anyone will describe the best protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). I have to do in cardiac cells(primary and...
23 June 2023 755 0 View
Hi everyone Can anyone will describe the best protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). I have to do in cardiac cells. Can anyone...
19 June 2023 4,910 0 View
Hello Everyone I have overexpressed one gene in cardiac cells. I notice so MVBs but to confirm that those are MVBs, please can anyone suggest me different markers which I can used to do further my...
06 June 2023 4,943 3 View
Hello Everyone I have overexpressed one gene in cardiac cells. I notice so MVBs but to confirm that those are MVBs, please can anyone suggest me different markers which I can used to do further...
05 June 2023 6,286 0 View
I have overexpressed one gene, and I noticed that whole MVBs are releasing out, instead of forming the bud with the plasma membrane and releasing the stored material. Could someone suggest to me...
03 June 2023 218 0 View
Please can any body guide my how to apply for fee reduction for publishing my research paper in journal PLOS ONE?
31 December 2021 976 6 View
Do there is always an intrinsic uncertainity for quenching data . How can we ensure that our data is correct ?
11 April 2021 5,608 0 View
I am using lipase of aspergillus niger for lipase inhibiton assay against some molecules. using substrate olive oil. Kindly if anybody have protocol? Thanks
13 February 2018 4,081 3 View
I purchased lipase. It is not soluble in water. After doing dialysis, scattering and aggregation has been noticed in UV spectra . What should I do to prevent the above same?
31 October 2017 133 2 View
During binding studies the fluorescence measurement of proteins on adding ligand is not showing one pattern.it is firstly decreasing and then start increasing with increase in wavelegnth.
23 March 2017 7,971 4 View