We work with human TEC, so I unfortunately don't have a mouse protocol to give you, but I know that the Hogquist group published a detailed protocol with video in the Journal of Visualised Experiments, that could help you:
http://www.jove.com/video/51780/isolation-identification-purification-murine-thymic-epithelial
This will work
Protocol for isolation of cells from lymph nodes
Starting material – isolated lymph nodes in PBS + 2% FBS
Protocol:
1. Put fresh 40 um cell strainer on 50 ml Falcon tube
2. Wash the strainer with 1 ml of PBS + 2% FBS
3. Put the lymph node on the strainer and push the cells through it using back of 1 ml syringe
4. Wash the cell strainer and the back of syringe with 2 ml of PBS + 2% FBS
5. Centrifuge the cells at 200 g for 10 min at 4°C and remove the supernatant
6. Resuspend the cells in 1 ml of PBS + 2% FBS and filter the cell suspension through 40 um cell strainer placed on 5 ml falcon tube
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