Kindly tell me what to do karyotype from leaf sample of Santalum album. The cell does not goes into swollen condition and chromosome also not get swollen and cell are not get swollen also.
You may increase length of time for pretreatment. You may as well try root tip preparation, since karyotype desire.
It expected that leaf preparation can be impaired with oil due to the presence of the cuticles. You may have to dewax before proceeding to other stage.
Sir, kindly tell me how to dewax the sample. Sir, root is not present in my sample Haustoria is present but it is a time being process to form young haustoria in tissue culture.
Who will tell you what to do if there is too much oil on the glass slide during the analysis of the leaf karyotype and the cells also do not swell?
If you want to see the chromosomes, there is a specific method for making temporary preparations. Want to see leaf chromosomes? Or want to see the chromosomes at the root tip of your plant. In this case, it is necessary to germinate the seed and crush the tip of the root according to a well-known method (search on the Internet). You won't see chromosomes on a leaf. Rather, you can try to prepare crushed preparations from the tip of the leaf. Read somewhere cytogenetic methods for analyzing plant chromosomes.
Respected L.I. Weisfeld
I am trying to accomplish my work from leaf tip but the cell does not goes in to swollen condition thats why I cant see chromosome. Kindly tell me where I am doing the mistake?
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