In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.
Why is there no peak shift in FTIR-ATR after the amine-modified polystyrene nanoplastics (PS-NH2) adsorption onto the NIPAAM/CMC hydrogel materials? I have repeated the tests, but I have gotten...
09 June 2024 9,007 6 View
The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.
01 June 2024 7,419 0 View
What is the significance of the -ve 'b' value or -ve intercept for the linear Langmuir isotherm plot?? I recently plotted some of my experimental data in a Langmuir linear isotherm plot. I have...
18 May 2024 4,576 10 View
In EFM measurement, we get topography and Phase image. From phase image what we can conclude? Let say, with thickness of a nanosheet phase is increasing. What does it mean that with thickness...
08 May 2024 7,590 1 View
Any lead will be helpful
24 April 2024 5,987 3 View
The instrument provided data of the IR spectrum is in %T vs wave number. But the peak is in the 3600 to 2600 cm-1 shows more than 100% transmittance. What are the probable reasons behind it? How...
21 April 2024 1,745 1 View
I have been growing HeLa to 90-100% confluency in 12 well dish, prior to subjecting them to heat stress at 43 degrees Celsius for 1.5hrs in a water bath. Unlike HEK293T, the HeLa cells lose...
16 April 2024 7,328 0 View
Actually, the Zeta potential measurement instrument (Zeta Sizer, DLS method) needs the refractive index and absorbance values of the hydrogel beads. How can I measure these two parameter values...
28 March 2024 6,947 4 View
Catalase assay for bacteria - not screening test with hydrogen peroxide The complete procedure using spectrophotometer
13 January 2024 8,828 1 View
Generally, the daily rainfalls are recorded at fixed time intervals (e.g., from 9 am to 9 am). We can find such records of historical rainfalls for centuries. However, in such records, even the...
20 November 2023 5,759 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...
30 July 2024 8,707 1 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View