In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.
I am trying to perform Gaussian optimization of a polymeric structure with 250 atoms. It is made up of only C and H atoms with several aromatic phenyl ring interlinked by C=C. Every time I am...
04 January 2021 9,616 2 View
I am required to do a project where I have to calculate discharge of streams/ watersheds in a high altitude area with extremely treacherous terrain. Will it be possible to achieve this objective...
27 December 2020 9,101 5 View
I understand the difference between NAAS score and Impact Factor but is it okay to publish in a non-NAAS, high IF journal? How will it be valued in ICAR interviews and related recruitments?
10 September 2020 7,575 3 View
I have below model: X = A*B Where A = a list of values with Lognormal distribution (size = 13) and B = another list of values with Lognormal distribution (size = 13) How can I perform 1st...
05 July 2020 1,963 1 View
We are missing some mass-spec raw files from 2017 and 2018 when deposition of raw files were not mandatory. Since most of the journals now require raw file availability through ProteomeXchange,...
14 June 2020 6,064 1 View
Good morning every body, i need books, Article, any other forms of materials about indian women in medicine, history of indian physicians
30 May 2020 9,218 1 View
I am working on rice root knot nematode. For identification purpose mostly second stage juveniles are used. I wonder why females or males are not used commonly for the same purpose? I am using...
26 March 2020 7,419 3 View
I am looking for a cheaper TagMan master mix for qPCR. I use FAM labeled primers for gene expression, does anyone have any suggestions
17 March 2020 1,105 2 View
In our case the Bejan number decreases with the increase of mass flow rate or Reynolds number of the multiphase fluids through a packed bed. However, if mass flow rate increases Nusselt number,...
27 December 2019 6,088 3 View
A function $f\in X^{[a, b]}$ is said to be Riemann-Pettis integrable if $x^* f$ is Riemann integrable for each $x^*\in X^*$ and if for each interval $I\subset [a, b]$, there is a vector in $x_I\in...
27 December 2019 7,532 9 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View