You could try some practical approach with BioAnalyzer kits for DNA and RNA. Another option is using a homebrew fluorometric strategy with specific DNA stains as methyl green (see linked file) and for RNA SYBR green II or pyronin.

Best,

Daniel

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Mr.  Pattnaik, after isolation of nucleotides, I was doing biospectrometer for quantification...... And I got the nucleotide concentration in microgram per ml.... But I hv one doubt that how can I convert this results (microgram per ml)  to microgram per mg of tissue.... Let's say I got the reading... 430 microgram per ml.... (Initially taking 20 mg muscle tissue for DNA isolation),  but I want to get the results of nucleotide, say DNA (Concentration said above) in microgram per mg tissue... Actually I don't understand how to get DNA concentration... in microgram per mg of tissue though I get DNA concentration in microgram per ml... 

As a relatively crude, but straightforward approach, I would suggest extracting DNA and RNA separately using the PCI and Trizol protocol, as suggested by others.

To calculate the concentration of, e.g., µg DNA per mg tissue, first note down the amount of tissue that you used in your extraction (e.g., 20 mg), and secondly calculate the total DNA yield: concentration of final extract * final extract volume (µg/ml * ml = µg). Your concentration of nucleic acid per tissue is then the quotient of yield over the initial tissue mass.

Do this for both DNA and RNA separately, and you can calculate DNA:RNA ratios, assuming that the extraction efficiency is roughly the same for both methods. Make sure to remove DNA contamination in your RNA extract (e.g., DNase treatment) and vice versa.

Good luck.

Thank you so much..