Dose any one has a Helicobaceter pylori avirulent or mild pathogenic starin?

Dear Researcher, We are looking for some mild pathogen/ non pathogen of Helicobacter pylori. We need to do some experiment for understanding of association of H. pylori with gastric...

08 September 2019 2,359 5 View

Can anybody please share protocol for synthesis of silver nanoplates (trangular and hexagonal)?

Can anybody please share protocol for synthesis of silver nanoplates (trangular and hexagonal)?

09 October 2016 9,687 4 View

Can anyone please suggest me a method for synthesizing nanoporous polysaccharide nanomaterial?

For my work I need a porous carbohydrate nanomaterial. Can anyone please suggest me a method for the same? Thanks

31 December 2015 4,228 4 View

How do I release cysteine from the surface of gold nanoparticles?

10 November 2014 9,855 5 View

I need to form polypeptide bonds at rt in aqueous medium. Do you have any suggestions how to do this?

I have a system, in which amino acids are bound to the matrix (with C-alpha-R) group. All amino acids have free amino and carboxylic groups. I need to add one layer of amino acids on the top of...

05 June 2013 2,515 3 View

How to induce necrosis in tumor tissue in vivo?

I would like to induce tumor necrosis in vivo in experimental mice model. Can anyone tell me how can I induce specifically tumor necrosis (not apoptosis) in mice. I don't have a laser facility to...

10 November 2012 3,770 3 View

How can I protect a preformed Bare Gold Nanorod with CTAB?

I have bare gold nanorods. I need to protect them with CTAB. Can anybody please suggest me a method for that?

07 August 2012 2,987 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View