Can any one please share vector to knock down a gene in Lactobacillus reuteri ?

06 June 2018 0 9K Report

Dose anyone has a vectro (any vector) which may be good for knock down a gene in Lactobacillus reuteri. If you have can you please share? I am in a real need for the same.

Badges
Science topic

More Diptiman Choudhury's questions See All

Dose any one has a Helicobaceter pylori avirulent or mild pathogenic starin?

Dear Researcher, We are looking for some mild pathogen/ non pathogen of Helicobacter pylori. We need to do some experiment for understanding of association of H. pylori with gastric...

08 September 2019 2,485 5 View

Can anybody please share protocol for synthesis of silver nanoplates (trangular and hexagonal)?

09 October 2016 9,822 4 View

Can anyone please suggest me a method for synthesizing nanoporous polysaccharide nanomaterial?

For my work I need a porous carbohydrate nanomaterial. Can anyone please suggest me a method for the same? Thanks

31 December 2015 4,363 4 View

How do I release cysteine from the surface of gold nanoparticles?

I have spherical gold nanoparticles bound to cysteine. I need to release the cysteine from the surface. Can anybody please suggest me a method to do that?

10 November 2014 9,972 5 View

I need to form polypeptide bonds at rt in aqueous medium. Do you have any suggestions how to do this?

I have a system, in which amino acids are bound to the matrix (with C-alpha-R) group. All amino acids have free amino and carboxylic groups. I need to add one layer of amino acids on the top of...

05 June 2013 2,638 3 View

How to induce necrosis in tumor tissue in vivo?

I would like to induce tumor necrosis in vivo in experimental mice model. Can anyone tell me how can I induce specifically tumor necrosis (not apoptosis) in mice. I don't have a laser facility to...

10 November 2012 3,895 3 View

How can I protect a preformed Bare Gold Nanorod with CTAB?

I have bare gold nanorods. I need to protect them with CTAB. Can anybody please suggest me a method for that?

07 August 2012 3,110 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View