How to optimise dcfda method?

I am using dcfda method to measure ros, where control cells are giving more fluorescence than the treatment. Does this mean the extract (as it is a potential antioxidant as observed by doph, frap...

31 December 2018 6,124 0 View

Where can I find HRMS (high-resolution mass spectrometry) facility for outsourcing samples?

02 March 2017 577 0 View

Which fungi is this?

can any one help by identifying this fungi which was isolated from contaminated workspace

31 December 2016 6,258 15 View

Can anyone help me to optimize bile concentration for invitro digestion model?

09 October 2016 4,013 3 View

Can any one help me with complete profiling of plant extract (aqueous)?

I have a plant extract [aqueous] which has shown positive results for qualitative tests for phenolics and sugars. Though the sample is showing good HPLC profile [mobile phase-acetonitrile:...

09 October 2016 1,804 11 View

If I am not sequencing the DGGE product, is it necessary to use forward primer with GC clamp?

02 March 2016 4,101 3 View

What is the difference between Caco-2 and Ht-29 cell lines?

05 June 2015 7,727 5 View

Can any one help with Identification of lactic acid bacterial isolates using genus specific primer?

I have an ATCC culture said to be L. plantarum and also 7 isolates which are tentatively characterized as lactic acid bacteria. when I am using genus specific primers for PCR, 3 isolates are...

03 April 2015 337 2 View

Can any one help me to separate only polyphenols from crude plant extract?

01 February 2015 9,395 14 View

Can anyone help with bacterial identification?

I have isolated one gram positive, catalase and oxidase negative rod shaped bacteria, which are in chains. Their size is large than the usual rods (when observed under 100X, length 3-4 times more...

07 August 2014 8,816 33 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View