I've had good luck using strong anion exchange (SAX) columns for this purpose. They will capture acidic compounds and let basic and neutral compounds elute. I find it to be a good initial clean-up step.

For information on this technique, please see:

http://www.isco.com/WebProductFiles/Applications/101/Poster_and_Paper_Reprints/Purification_of_Phenolic_Flavonoids.pdf

http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN98_Hints%20for%20Strong%20Ion%20Exchange%20Resins.pdf

http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN87_RediSep%20Rf_SAX_Column_Applications.pdf

Dear,

you can run column chromatography using solvents of suitable polarity and run TLC or HPLC to check the purity of isolated compound.

It is very complicated, you can try using SPE C-18, but I doubt you recover 100% polyphenols...

luck

Regards

simple method of extraction of polyphenolic compound is that dissolve your crude extract in water and the make 60%ethylacetate/hexane solution and the add it in extract it will extract upto 80%polyphenolics.

I would suggest, first a liquid-liquid extraction or partitioning water-hexane of your material and then use ethyl-acetate with the water phase in order to get the flavonoids and most of the phenolics in the sample.  There are a lot of references on natural products chemistry for that purpose.

U Didn't mention the quantity required /purpose of your study. Still the Simple method is Preparative TLC, Plese refer to http://www.tandfonline.com/doi/abs/10.1080/10826070600574911?journalCode=ljlc20#.VNyxsVLhWt8 (Claudia Cimpoiu. 2006. Analysis of Some Natural Antioxidants by Thin‐Layer Chromatography and High Performance Thin‐Layer Chromatography Journal of Liquid Chromatography & Related Technologies. 29(7-8). 1125-1142.

there are  lot of papers available on this...

best of luck

Dr kkk

I use boiling with methanol of dried plant material, but I get a complex mixture.

Thank you all

i don't want to use solvents other that water for this. In this water extract sugars are also present which i want to remove. please if someone knows answer me

TLC of secondary metabolites: plant secondary metabolites are naturally  producing compounds, which are not of earliest importance to plants, on other sides, they are subordinate means they are not involved in original metabolism. Secondary metabolites are produced on external stimuli, abiotic stress biotic stress, injury, heat, cold, etc. some of the important secondary metabolites are alkaloids, esters, flavonoids, isoflavonides, Phytoalexins, Tannins, Salicylic acid, lignin. All these metabolites are identified from plant explants by using TLC. Stress conditions on plant's results in production of secondary metabolites from plant such as amino acid example proline, quaternary amines such as poly amine, different sugar and alcohol. All these secondary metabolites could be analyzed using TLC. Different kinds of the metabolites are used for formulation of medicines and cosmetology. Secondary metabolites isolated and purified may be used for antimicrobials test, compound structure identification, MS, FTIR, and GCMS. It can also be used for improving organic farming for better crop results

http://www.rjpbcs.com/pdf/2014_5%284%29/%5B53%5D.pdf

Try TLC with butanol: acetic acid: water (4:1:1). or Ethyl acetate: Formic acid: Acetic acid : Water.

Most of the polyphenolic are UV light sensitive and give fluorescence. 

Sometime NP-PEG or 5 % KOH or 5 % FeCl3 is used for detection.

First of all, you may need to detect the presence of polyphenolics in the crude extract by TLC, possibly easy with reverse phase TLC since they are polar. Secondly, you may use petroleum ether or hexane to remove nonpolar compounds (petroleum ether better). Then, use diethyl ether to separate tannins (also polyphenolics) by dissolving extract in small volume of methanol and add with diethyl ether. Tannins will suddenly precipitate. Diethyl ether fraction of course still contains phenolics, which can be further separated by using C-18, roughly with MeOH:CHCL3 (9:0.1).

Another step is centrifugation.

Pl. check whether the attached information is of any use to you.

Best wishes.

The adsorption characteristics of tea polyphenols onto three polymeric adsorbents with amide group. PMVBA (poly (N-methyl-N-pvinylbenzylacetamide)), PMVBU (poly (N-methyl-N-p-vinylbenzylurea)), and PMA (poly (N-methylacrylamide)) were used as adsorbent. Based on the results of adsorption isotherms and adsorption kinetics experiments, PMVBU was chosen as the best resin for adsorption capacity and adsorption enthalpy for tea polyphenols. Adsorption mechanism suggests that multiple hydrogen bonding and hydrophobic interaction are included adsorption of tea polyphenols onto the three adsorbents in aqueous solution

Huang J., Huang K., Liu S., Luo Q., Xu M., ‘Adsorption Properties of Tea Polyphenols Onto Three Polymeric Adsorbents with Amide Group’,Journal of Colloid and Interface Science, 2007, 315, 407–414.

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Pp37

Adsorption studies of polyphenols and their attributes.

• ADSORPTION SYSTEMS
• EXPERIMENTAL SETUP
• ANALYTICAL TECHNIQUE
• ATTRIBUTES

Alihan KARAKAYA (2011), PURIFICATION OF POLYPHENOLIC COMPOUNDS FROM CRUDE OLIVE LEAF EXTRACT, Izmir Institute of Technology

https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=33&ved=0CC4QFjACOB4&url=http%3A%2F%2Flibrary.iyte.edu.tr%2Ftezler%2Fmaster%2Fkimyamuh%2FT000893.pdf&ei=P_n_VIieIovzUrn0gOAG&usg=AFQjCNElHtjJNIPSG8UAyVkw_WJC9ZvO_g

Thank you al