Shubha Likitha

12 Questions 15 Answers 0 Followers

Questions related from Shubha Likitha

How to optimise dcfda method?

I am using dcfda method to measure ros, where control cells are giving more fluorescence than the treatment. Does this mean the extract (as it is a potential antioxidant as observed by doph, frap...

01 January 2019 6,171 0 View

Where can I find HRMS (high-resolution mass spectrometry) facility for outsourcing samples?

I am in search of HRMS (high-resolution mass spectrometry) facility for complete profiling of plant extract, can anyone help me with the facility. What will be the approximate cost per sample?

03 March 2017 628 0 View

Which fungi is this?

can any one help by identifying this fungi which was isolated from contaminated workspace

01 January 2017 6,313 15 View

Can any one help me with complete profiling of plant extract (aqueous)?

I have a plant extract [aqueous] which has shown positive results for qualitative tests for phenolics and sugars. Though the sample is showing good HPLC profile [mobile phase-acetonitrile:...

10 October 2016 1,856 11 View

Can anyone help me to optimize bile concentration for invitro digestion model?

I want to use Bile salt (Sigma 48305) for invitro digestion model, which I want to further subject to invitro fermentation. What concentration of above mentioned bile salt can be used for...

10 October 2016 4,106 3 View

If I am not sequencing the DGGE product, is it necessary to use forward primer with GC clamp?

I am thinking to use 338F and 518R primers for DGGE analysis of bacterial consortium. Please suggest me the suitable primers, and is it necessary to use GC clamp for forward primer if I am not...

03 March 2016 4,144 3 View

What is the difference between Caco-2 and Ht-29 cell lines?

I am confused about these two cell lines- Caco2 and HT 29. both seems to be similar but compound that causes antiproliferation & apoptosis in caco2 means its harmfull to colon whereas...

06 June 2015 7,778 5 View

Can any one help with Identification of lactic acid bacterial isolates using genus specific primer?

I have an ATCC culture said to be L. plantarum and also 7 isolates which are tentatively characterized as lactic acid bacteria. when I am using genus specific primers for PCR, 3 isolates are...

04 April 2015 392 2 View

Can any one help me to separate only polyphenols from crude plant extract?

I have crude plant extracts of which i need to separate out only polyphenols ( get rid of sugars and others).

02 February 2015 9,448 14 View

Can any one help with phenolic acid decarboxylase enzyme identification?

By activity staining method.

08 August 2014 6,112 3 View

Can anyone help with bacterial identification?

I have isolated one gram positive, catalase and oxidase negative rod shaped bacteria, which are in chains. Their size is large than the usual rods (when observed under 100X, length 3-4 times more...

08 August 2014 8,868 33 View

Where can I get chemical-4 ethyl phenol & 4 vinyl phenol?

I want to procure these chemicals for my work. These are phenolic acid degradation products.

05 May 2014 7,697 1 View