How to optimise dcfda method?

I am using dcfda method to measure ros, where control cells are giving more fluorescence than the treatment. Does this mean the extract (as it is a potential antioxidant as observed by doph, frap...

31 December 2018 6,124 0 View

Which fungi is this?

can any one help by identifying this fungi which was isolated from contaminated workspace

31 December 2016 6,258 15 View

Can anyone help me to optimize bile concentration for invitro digestion model?

09 October 2016 4,013 3 View

Can any one help me with complete profiling of plant extract (aqueous)?

I have a plant extract [aqueous] which has shown positive results for qualitative tests for phenolics and sugars. Though the sample is showing good HPLC profile [mobile phase-acetonitrile:...

09 October 2016 1,804 11 View

If I am not sequencing the DGGE product, is it necessary to use forward primer with GC clamp?

02 March 2016 4,101 3 View

What is the difference between Caco-2 and Ht-29 cell lines?

05 June 2015 7,727 5 View

Can any one help with Identification of lactic acid bacterial isolates using genus specific primer?

I have an ATCC culture said to be L. plantarum and also 7 isolates which are tentatively characterized as lactic acid bacteria. when I am using genus specific primers for PCR, 3 isolates are...

03 April 2015 337 2 View

Can any one help me to separate only polyphenols from crude plant extract?

01 February 2015 9,395 14 View

Can any one help with phenolic acid decarboxylase enzyme identification?

By activity staining method.

07 August 2014 6,057 3 View

Can anyone help with bacterial identification?

I have isolated one gram positive, catalase and oxidase negative rod shaped bacteria, which are in chains. Their size is large than the usual rods (when observed under 100X, length 3-4 times more...

07 August 2014 8,816 33 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View