How to calculate Unknown protein concentration based on standard curve by using BSA method?

I made curve for standard protein of BSA 125-1000 ug/ml. I draw linear line in Excel I got m value and c value. which can be generated by plotting the std conc in Xaxis and absorbance in Y in...

06 July 2019 8,443 1 View

What is the best housekeeping genes in humantissue and blood samples for qpcr analysis?

11 December 2016 5,797 8 View

Can any one use HPV primers GP5+ and GP6+ ?

04 May 2016 2,992 3 View

Can anybody suggest hpv (human papiloma virus) L1 region primers along with pcr conditions?

01 February 2016 9,372 2 View

What is the best DNA extraction method for NGS Analysis?

01 February 2016 3,535 7 View

How to adapt the cells from RPMI TO DMEM medium?

I work with Hella in RPMI 1640. I need to change the medium of this cells from RPMI to DMEM. How to change these cell lines to adapt the cells to the new medium?

01 February 2014 9,739 13 View

How to avoid hairpin loops in my PCR primers?

How to avoid hairpin loops in my pcr primers?

09 October 2013 9,241 7 View

Can anyone recommend any articles on plant compound-induced phosphorylated p53 expression in cell lines detected by flow cytometry?

Can anyone recommend any articles on plant compound-induced phosphorylated p53 expression in cell lines detected by flow cytometry?

09 October 2013 3,931 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View