in our gene have 7-8 rep of GGC we would like to observe these repeats in patients samples .how to perform Gene scan ,and how to design primer for specially gene scan analysis
I made curve for standard protein of BSA 125-1000 ug/ml. I draw linear line in Excel I got m value and c value. which can be generated by plotting the std conc in Xaxis and absorbance in Y in...
06 July 2019 8,560 1 View
we are working on cervical cancer ,we would see the expression levels of target genes in both control and patient samples. we are planning to use internal control as Coiled-coil-helix-coiled...
11 December 2016 5,929 8 View
Explain PCR conditions for these primers
04 May 2016 3,087 3 View
When I used gp5 and gp6 primers my product length was 150 bp ,according that i got it bands, but even i got band in non template control. After then i use everything fresh (pcr mix ,water) even...
01 February 2016 9,459 2 View
I would like to know what the best DNA extraction method for NGS analysis generally we use phenol chloroform method for Cancerous tissue DNA isolation but i heard phenol inter pt the NGS...
01 February 2016 3,634 7 View
I work with Hella in RPMI 1640. I need to change the medium of this cells from RPMI to DMEM. How to change these cell lines to adapt the cells to the new medium?
01 February 2014 9,841 13 View
How to avoid hairpin loops in my pcr primers?
09 October 2013 9,424 7 View
Can anyone recommend any articles on plant compound-induced phosphorylated p53 expression in cell lines detected by flow cytometry?
09 October 2013 4,039 0 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View