I made curve for standard protein of BSA 125-1000 ug/ml.

I draw linear line in Excel I got m value and c value. which can be generated by plotting the std conc in Xaxis and absorbance in Y

in the equation Y=mX+C, Here i got zero(0) m value then how to calculate unknown sample concentration based on standard curve.

Here i attach the Excel.

Similar questions and discussions