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Questions related from Deepthi Pasumarthi
I made curve for standard protein of BSA 125-1000 ug/ml. I draw linear line in Excel I got m value and c value. which can be generated by plotting the std conc in Xaxis and absorbance in Y in...
07 July 2019 8,536 1 View
we are working on cervical cancer ,we would see the expression levels of target genes in both control and patient samples. we are planning to use internal control as Coiled-coil-helix-coiled...
12 December 2016 5,904 8 View
in our gene have 7-8 rep of GGC we would like to observe these repeats in patients samples .how to perform Gene scan ,and how to design primer for specially gene scan analysis
06 June 2016 687 0 View
Explain PCR conditions for these primers
05 May 2016 3,057 3 View
When I used gp5 and gp6 primers my product length was 150 bp ,according that i got it bands, but even i got band in non template control. After then i use everything fresh (pcr mix ,water) even...
02 February 2016 9,429 2 View
I would like to know what the best DNA extraction method for NGS analysis generally we use phenol chloroform method for Cancerous tissue DNA isolation but i heard phenol inter pt the NGS...
02 February 2016 3,608 7 View
I work with Hella in RPMI 1640. I need to change the medium of this cells from RPMI to DMEM. How to change these cell lines to adapt the cells to the new medium?
02 February 2014 9,809 13 View
How to avoid hairpin loops in my pcr primers?
10 October 2013 9,380 7 View
Can anyone recommend any articles on plant compound-induced phosphorylated p53 expression in cell lines detected by flow cytometry?
10 October 2013 4,000 0 View
