How to calculate Unknown protein concentration based on standard curve by using BSA method?

I made curve for standard protein of BSA 125-1000 ug/ml. I draw linear line in Excel I got m value and c value. which can be generated by plotting the std conc in Xaxis and absorbance in Y in...

06 July 2019 8,443 1 View

What is the best housekeeping genes in humantissue and blood samples for qpcr analysis?

11 December 2016 5,797 8 View

Can any one explain Gene scan analysis?

05 June 2016 616 0 View

Can anybody suggest hpv (human papiloma virus) L1 region primers along with pcr conditions?

01 February 2016 9,372 2 View

What is the best DNA extraction method for NGS Analysis?

01 February 2016 3,535 7 View

How to adapt the cells from RPMI TO DMEM medium?

I work with Hella in RPMI 1640. I need to change the medium of this cells from RPMI to DMEM. How to change these cell lines to adapt the cells to the new medium?

01 February 2014 9,739 13 View

How to avoid hairpin loops in my PCR primers?

How to avoid hairpin loops in my pcr primers?

09 October 2013 9,241 7 View

Can anyone recommend any articles on plant compound-induced phosphorylated p53 expression in cell lines detected by flow cytometry?

Can anyone recommend any articles on plant compound-induced phosphorylated p53 expression in cell lines detected by flow cytometry?

09 October 2013 3,931 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View