I have to selectively isolate Endophytic Actinomycetes from a plant . In order to prevent the growth of fungi and bacteria, in papers , Antibiotics are added. Should that be added Before / After sterilization of media ?
Dear Moni Philip Jacob K
Pls. find the attached files, for several purpose, may help in your research , good luck
http://edenrcn.com/protocols/Individual%20Protocols/Square_Protocol.pdf
http://www.madsci.org/~lynn/micro/media/antibiotics.html
http://2015.igem.org/wiki/images/6/6c/TU_Eindhoven_Protocols_Preparation_of_general_necessities.pdf
http://hymanlab.mpi-cbg.de/hyman_lab/wp-content/uploads/2012/08/atcc_recommendations_antibiotic_concentrations.pdf
http://www.sci.sdsu.edu/~smaloy/Research/pdf%20files/Antibiotics.pdf
http://www.strgen.org/protocols/studier.pdf
http://www.jove.com/video/3064/aseptic-laboratory-techniques-plating-methods
http://www.rpgroup.caltech.edu/courses/aph162/2007/Protocols/Size-rate%20protocols.pdf
https://www.normalesup.org/~vorgogoz/protocols/yeast-protocols/YeastMediaRecipes.pdf
http://web.mnstate.edu/provost/media%20preparation%20protocol.pdf
Regards
Prof. Houda Kawas
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