We are analysing the expression of Afp (endoderm marker), Sma (mesoderm) and Tuj1 (ectoderm) by mESCs that have been cultured in spontaneous differentiation conditions for 10 days with immunofluorescence staining, and we are detecting tha signal for more than one marker on several cells at the same time, i.e. we observe cells that are positive for Afp and for Tuj1. Is it possible that a single cell expresses more than one of these markers at once?
Dino Santos Matias
Hi Ot Massafret,
Spontaneous differentiation is a rather chaotic process which can lead to several intermediate states. Is this occurring in vivo or in vitro? I’d suggest you let that differentiation run for longer and see if you still see those mixed/intermediate phenotypes.
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