Ot Massafret

5 Questions 1 Answers 0 Followers

Questions related from Ot Massafret

Can a differentiating mouse embryonic stem cell express ectoderm, mesoderm and endodem markers at the same time?

We are analysing the expression of Afp (endoderm marker), Sma (mesoderm) and Tuj1 (ectoderm) by mESCs that have been cultured in spontaneous differentiation conditions for 10 days with...

05 December 2022 7,484 1 View

Could someone help me identify these structures observed on spontaneously differentiating mouse embryonic stem cells in this SEM image?

We took an image of differentiating mESCs with SEM and observed these structures all over the cells in some regions of the coverslip. The structures are about 700 nm long. Any idea of what could...

03 November 2022 4,587 4 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

17 February 2021 8,576 3 View

Which is the best way to calculate doubling time for human embryonic stem cells?

Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...

11 February 2021 3,044 4 View

What's the most optimal design for a qPCR experiment with 10 samples and 16 genes?

Hi! I have to perform a qPCR experiment in which I would like to evaluate the expression of 13 genes (plus 3 HKG) on 10 samples. I must carry the runs on a 96-well qPCR plate since I need to...

15 November 2020 5,651 4 View