Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Which is the best way to calculate doubling time for human embryonic stem cells?

Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...

10 February 2021 2,895 4 View

What's the most optimal design for a qPCR experiment with 10 samples and 16 genes?

Hi! I have to perform a qPCR experiment in which I would like to evaluate the expression of 13 genes (plus 3 HKG) on 10 samples. I must carry the runs on a 96-well qPCR plate since I need to...

14 November 2020 5,530 4 View

Does anybody know of a density dip in the axis of symmetry in a free jet expansion and the cause for it?

In a reconstruction of the density profile of an atomic gas jet emanating from an axis-symmetric converging microscopic nozzle, we got from the Abel inversion of some slices a dip in the density...

15 December 2015 5,718 2 View

Can someone show me the derivation to the width of the potential barrier for tunneling of an electron in the presence of strong EM wave?

can someone please show me the derivation to the width of the potential barrier w=Ip/(e*E), where Ip is the ionization enery, for electron tunneling in the presence of strong electronmagnetic...

03 December 2014 1,177 1 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

How much of the bird trade does CITES cover?

Trade in wild-caught birds is big business. Most data on which species are traded and in what volumes comes from CITES. But the CITES Appendices do not cover all traded bird species, e.g. it does...

30 November 2020 1,955 4 View

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23 November 2020 6,277 5 View

Anyone an expert in algae in Victoria, Australia?

I found this (very pretty) algae (see picture) in a bore-fed and suburban catchment run-off fed, human made lake (Newport Lakes) in Newport, Victoria, Australia. I'm a vertebrate zoologist with a...

09 November 2020 7,587 3 View

Indicated hydrogens in heterocyclic compounds - nomenclature?

Hello, I have a question to the IUPAC nomenclature of heterocyclic compounds. Usually hydrogen atoms in the ring system not attached to atoms connected by double bonds are denoted as indicated...

03 November 2020 3,313 3 View

Who is conducting research on the organization of gardens using mulching agrotechnology in arid areas?

In order to carry out sustainable agriculture in arid regions, it is necessary to use water efficiently, use water-saving agro-technologies. One of the water-saving agro-technologies in...

27 October 2020 2,889 3 View

Do you agree to modify the Plagiarism rules?

The plagiarism which we are checking with the help of Turnitin software counts the similarity of usual English words even helping verbs and proverbs. If the similarity of specific terminology is...

11 October 2020 3,235 6 View

What are your suggestions for getting a clear classification for orchids that can be easy for clear orientation?

Many books have been published containing pictures and measurements and nomenclatures for orchids. Looking at those pictures is something that confuse a lot getting you crazy. You can not...

07 October 2020 9,643 3 View

Do you think thesis reviewer should be allocated based on their subject knowledge?

Recently I received one master thesis on Six Sigma. It was referred to me for assessing the validity of the reviewer comments. The student raised some questions on the review comments and quality...

01 September 2020 7,596 5 View