How to calculate sample concentration and percentage by using AUC of sample and AUC of standard?
I ran standard and samples at same conditions in HPLC and have good resolution of peaks.
Can anybody help me in this regard?
Can anybody provide me standard methods for the formulation and evaluation of herbal anti-aging gel, cream and serum with accurate formula? My goal is to develop three (as said above) individual...
03 April 2019 8,057 4 View
Is anyone working in the chemistry of Indigo blue dye who suggest me the possibilities to get indoxyl glycoside (Indican) from blue indigo dye? I mean i want to do reverse process i.e. conversion...
04 May 2018 3,728 0 View
Can any body tell me how i correlate antioxidant activity with total phenol and express my results graphically??????????? Is it possible by using Microsoft excel sheet??? If yes can any one guide...
05 June 2015 1,608 1 View
I run a column of pet. ether extract and collected 25 fractions from pet.ether: ethyl acetate (80:20) mobile phase. One fraction showed single spot on TLC in same solvent system but difficult to...
04 May 2014 652 10 View
I extracted plant material with ethanol for anticonvulsant activity. DMSO is used to dissolve the ethanolic extract. But ethanol itself has CNS stimulant activity. So please suggest a method by...
31 December 2013 1,773 11 View
I have added silica gel in excess amount for column chromatography in methanol extract. Can anyone tell me, can this create any problem during running of column? If yes, what is the solution?
10 November 2013 5,227 7 View
Is mass spectrum helpful in clearly identification of compound?
02 March 2013 3,212 2 View
Using Preparative tlc. I elute two fractions (Methanolic and Watery) from water extract of tubers of a plant. Both fractions are reddish color but in water fraction there is a small amount of...
02 March 2013 1,397 9 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View