Need methods for Formulation and evaluation of anti-aging cream, gel and serum?

Can anybody provide me standard methods for the formulation and evaluation of herbal anti-aging gel, cream and serum with accurate formula? My goal is to develop three (as said above) individual...

03 April 2019 7,993 4 View

Calculation of Sample concentration using AUC in HPLC?

How to calculate sample concentration and percentage by using AUC of sample and AUC of standard? I ran standard and samples at same conditions in HPLC and have good resolution of peaks. Can...

06 July 2018 3,472 0 View

How i make a relationship between antioxidant activity and total phenol content

05 June 2015 1,538 1 View

Can anyone suggest how I can crystallize the compounds from pet. ether fractions collected by CC?

I run a column of pet. ether extract and collected 25 fractions from pet.ether: ethyl acetate (80:20) mobile phase. One fraction showed single spot on TLC in same solvent system but difficult to...

04 May 2014 565 10 View

Can any one suggest how to determine the percentage of solvent present in any extract?

I extracted plant material with ethanol for anticonvulsant activity. DMSO is used to dissolve the ethanolic extract. But ethanol itself has CNS stimulant activity. So please suggest a method by...

31 December 2013 1,709 11 View

What happened when excess silica gel is added to the methanol extract before column chromatography?

I have added silica gel in excess amount for column chromatography in methanol extract. Can anyone tell me, can this create any problem during running of column? If yes, what is the solution?

10 November 2013 5,161 7 View

How do I crystallize compounds isolated through water extract by preparative tlc and how do I separate dissolved silica gel from water fraction?

Using Preparative tlc. I elute two fractions (Methanolic and Watery) from water extract of tubers of a plant. Both fractions are reddish color but in water fraction there is a small amount of...

02 March 2013 1,332 9 View

Can I perform LCMS or any other Instumental analysis of a watery eluted fraction which helps in identification of a particular compound?

Is mass spectrum helpful in clearly identification of compound?

02 March 2013 3,135 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

If a journal doesn't provide doi after acceptance of the manuscript, is it authentic?

It is related to submission of the manuscript in a Journal and the process of its online availability.

18 February 2021 9,269 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View