Calculation of Sample concentration using AUC in HPLC?

How to calculate sample concentration and percentage by using AUC of sample and AUC of standard? I ran standard and samples at same conditions in HPLC and have good resolution of peaks. Can...

06 July 2018 3,472 0 View

Can anyone working in the chemistry of blue dye obtained from Indigofera or other plant species?

Is anyone working in the chemistry of Indigo blue dye who suggest me the possibilities to get indoxyl glycoside (Indican) from blue indigo dye? I mean i want to do reverse process i.e. conversion...

04 May 2018 3,658 0 View

How i make a relationship between antioxidant activity and total phenol content

05 June 2015 1,538 1 View

Can anyone suggest how I can crystallize the compounds from pet. ether fractions collected by CC?

I run a column of pet. ether extract and collected 25 fractions from pet.ether: ethyl acetate (80:20) mobile phase. One fraction showed single spot on TLC in same solvent system but difficult to...

04 May 2014 565 10 View

Can any one suggest how to determine the percentage of solvent present in any extract?

I extracted plant material with ethanol for anticonvulsant activity. DMSO is used to dissolve the ethanolic extract. But ethanol itself has CNS stimulant activity. So please suggest a method by...

31 December 2013 1,709 11 View

What happened when excess silica gel is added to the methanol extract before column chromatography?

I have added silica gel in excess amount for column chromatography in methanol extract. Can anyone tell me, can this create any problem during running of column? If yes, what is the solution?

10 November 2013 5,161 7 View

How do I crystallize compounds isolated through water extract by preparative tlc and how do I separate dissolved silica gel from water fraction?

Using Preparative tlc. I elute two fractions (Methanolic and Watery) from water extract of tubers of a plant. Both fractions are reddish color but in water fraction there is a small amount of...

02 March 2013 1,332 9 View

Can I perform LCMS or any other Instumental analysis of a watery eluted fraction which helps in identification of a particular compound?

Is mass spectrum helpful in clearly identification of compound?

02 March 2013 3,135 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View