Need methods for Formulation and evaluation of anti-aging cream, gel and serum?

Can anybody provide me standard methods for the formulation and evaluation of herbal anti-aging gel, cream and serum with accurate formula? My goal is to develop three (as said above) individual...

03 April 2019 7,993 4 View

Calculation of Sample concentration using AUC in HPLC?

How to calculate sample concentration and percentage by using AUC of sample and AUC of standard? I ran standard and samples at same conditions in HPLC and have good resolution of peaks. Can...

06 July 2018 3,472 0 View

Can anyone working in the chemistry of blue dye obtained from Indigofera or other plant species?

Is anyone working in the chemistry of Indigo blue dye who suggest me the possibilities to get indoxyl glycoside (Indican) from blue indigo dye? I mean i want to do reverse process i.e. conversion...

04 May 2018 3,658 0 View

How i make a relationship between antioxidant activity and total phenol content

05 June 2015 1,538 1 View

Can anyone suggest how I can crystallize the compounds from pet. ether fractions collected by CC?

I run a column of pet. ether extract and collected 25 fractions from pet.ether: ethyl acetate (80:20) mobile phase. One fraction showed single spot on TLC in same solvent system but difficult to...

04 May 2014 565 10 View

Can any one suggest how to determine the percentage of solvent present in any extract?

I extracted plant material with ethanol for anticonvulsant activity. DMSO is used to dissolve the ethanolic extract. But ethanol itself has CNS stimulant activity. So please suggest a method by...

31 December 2013 1,709 11 View

What happened when excess silica gel is added to the methanol extract before column chromatography?

I have added silica gel in excess amount for column chromatography in methanol extract. Can anyone tell me, can this create any problem during running of column? If yes, what is the solution?

10 November 2013 5,161 7 View

How do I crystallize compounds isolated through water extract by preparative tlc and how do I separate dissolved silica gel from water fraction?

Using Preparative tlc. I elute two fractions (Methanolic and Watery) from water extract of tubers of a plant. Both fractions are reddish color but in water fraction there is a small amount of...

02 March 2013 1,332 9 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

What is the chemical composition of Cucumis melo roots?

I have found an old pharmaceutical research from 1833 in which emetic power of Cucumis melo roots was confirmed. I cannot find data about chemical constituents of the Cucumis melo roots. Their...

30 October 2020 3,633 3 View

How do you extract a specific chemical from a plant?

I know how to get the general extract from a plant - I can get the total phenols, alkaloids, Flavanoids, etc, but I want to isolate just one particular phenolic compound from the total extract....

21 October 2020 6,384 19 View

What is the best method and solvent for isolation of alkaloids from leaves?

my target to isolate alkaloids from plant leaves, as phytochemistry of plant reported for some alkaloid derivatives , so please guide me.

01 August 2020 7,376 15 View

Phytochemical : looking for Useful chemicals :List of some common and cheapest Chemical Reagent and instrument are mandatory for small Analysis Lab?

07 March 2020 8,329 2 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

Can anyone suggest the name or contact details of commercial laboratories offering chemical analysis of organic materials through LC-MS/MS?

I have plant methanol, ethyl acetate, n-hexane, and butanol extracts. I am interested in the chemical profiling of each extract through LC-MS/MS. If someone knows the details of commercial labs...

31 December 2019 1,508 4 View

Free or Low-cost Journal with High Impact Factor?

Hi, I want to publish a paper on phytochemistry, and I can't afford something like Elseveir Phytochemistry as it costs $3300+ taxes. So, please help me find a phytochemistry journal with high...

09 October 2019 9,421 3 View

How to patent a new protocole as US.patent ?

I have an open question that is not often asked, as a biochemist I'm working on phytochemistry field and have from time to time problems to solve, sometimes trying to solve a problem may lead to...

30 September 2019 6,382 4 View

Except literature search, how to know a chemical compound belong to which family?

I am writing a review of the herbal plants which requires to elaborate the phytochemistry information. I just wonder if there is any easier way to know which chemical compound belongs to which...

24 September 2019 7,868 3 View