Jan Trnka

3 Questions 14 Answers 0 Followers

Questions related from Jan Trnka

Is there a good, non-proprietary stop reagent for AmplexRed/HRP reaction?

How do you stop the oxidation of Amplex Red? Life Tech sell a proprietary stop reagent but I'd rather use something of which I know the composition. Any ideas? Strong acids will alter...

01 January 2014 9,452 2 View

How stable is ATP in a TCA or a PCA cell extract?

Is it possible to keep ATP (frozen or at RT) for a few hours or days, before measuring it with a luminescence kit?

08 August 2013 2,889 8 View

How do you convert SNARF fluorescence ratios to pH values (intracellular, confocal)?

The calibration method and formula given in the Mol. Probes product sheet makes no sense to me (gives infinite values for one of the calibration endpoints) and there is no explanation how to pick...

08 August 2013 3,931 13 View