I have stock siRNA solution of 20uM. I would like to calculate the final concentration of siRNA in 500ul DMEM and how much volume do I need to reach the final concentration, can anyone help me with this conversion and calculation?
Nobody can help you because the question is incomplete and make no sense. There is a vital information missing from the question.
Muna Al-Jabri What volume of stock solution of 20uM are you using? Then you can use the c1V1=c2V2 formula to find the answer to your question. (c1 and V1 refers to initial concentration and volume, c2 and V2 refers to final concentration and volume)
Agree with Hasan Issa , clear crystal answer cannot be given in this regard
What is your target concentration to treat the cells? Like, if you want to treat 100 nM concentration, the calculation will be ((100 nM/20 uM)*500 uL) = ((100 nM/20 x1000 nM)*500 uL) = 2.5 uL (of stock siRNA).
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