I have a problem with clear renal cell carcinoma cell lines Caki-I and Caki-II. I can not proliferate them. Are there anybody who studied these cell lines before? Can somebody give some advice. Because it is very critic for me.
Dear Emine!
Please You look at these articles:
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2.2. Cell Lines and Culture Conditions
The human ccRCC cell lines, Caki-1 (ATCC® HTB-46™) and Caki-2 (ATCC® HTB-47™) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were maintained in McCoy’s 5A (modified) medium supplemented with 10% FBS, 1% l-glutamine and 1% penicillin/streptomycin. Cells were cultured in a 37 °C humidified atmosphere containing 5% CO2 and 95% air. All methods were conducted in accordance with the relevant guidelines and regulations of the institutional biosafety committee.
Article Characterisation of the Morphological, Functional and Molecu...
The RCC cell lines 786-0, Caki-1 and Caki-2 were obtained from American Type Culture Collection (Rockville, MD). The RCC cell lines were cultured in DMEM/F12 (Gibco, Invitrogen, CA, USA) supplemented with foetal bovine serum (10%), penicillin (50 U/ml), streptomycin (50 μg/ml) and amphotericin B (0.125 μg/ml) in a humidified atmosphere of 5% CO2 in air at 37°C. All cell lines were recurrently tested and determined to be mycoplasma-free.
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