No title

03 April 2018 890 0 View

Does anybody know about ICI journals and their validation?

Does anybody know about ICI journals and their validation?

01 February 2018 365 0 View

Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this?

Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this? What structural difference(s) have made this possible?

04 May 2016 5,308 14 View

How to perform DNA\ase I footprinting?

Can anybody answer my question? You have determined the DNA-binding site of a protein by DNA footprinting after labeling one strand. To check your results, you repeat the experiment after labeling...

31 December 2013 3,806 1 View

cDNA pattern on gel electrophoresis

Does cDNA have a pattern on gel electrophoresis?

04 May 2012 3,846 0 View

Does DMSO, when used as a solvent, effect gene expression (at protein level) in cell culture?

I have treated my cells with two different drugs. One of these was disssolved in MeOH and the other in DMSO. In the control samples, we expected to see the same pattern (in western blotting), but...

03 April 2012 9,820 13 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View