Ca we filter the cell culture media containing Pen/Strep?

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How long can be kept the freeze dried plant extract at -20 °C?

How long can be kept the freeze dried plant extract, at -20 °C?

03 April 2018 961 0 View

Does anybody know about ICI journals and their validation?

01 February 2018 431 0 View

Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this?

Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this? What structural difference(s) have made this possible?

04 May 2016 5,420 14 View

How to perform DNA\ase I footprinting?

Can anybody answer my question? You have determined the DNA-binding site of a protein by DNA footprinting after labeling one strand. To check your results, you repeat the experiment after labeling...

31 December 2013 3,885 1 View

cDNA pattern on gel electrophoresis

Does cDNA have a pattern on gel electrophoresis?

04 May 2012 3,939 0 View

Does DMSO, when used as a solvent, effect gene expression (at protein level) in cell culture?

I have treated my cells with two different drugs. One of these was disssolved in MeOH and the other in DMSO. In the control samples, we expected to see the same pattern (in western blotting), but...

03 April 2012 9,994 13 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View