Does cDNA have a pattern on gel electrophoresis?
Ca we filter the cell culture media containing Pen/Strep?
05 June 2019 4,116 4 View
03 April 2018 890 0 View
Does anybody know about ICI journals and their validation?
01 February 2018 365 0 View
Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this? What structural difference(s) have made this possible?
04 May 2016 5,308 14 View
Can anybody answer my question? You have determined the DNA-binding site of a protein by DNA footprinting after labeling one strand. To check your results, you repeat the experiment after labeling...
31 December 2013 3,806 1 View
I have treated my cells with two different drugs. One of these was disssolved in MeOH and the other in DMSO. In the control samples, we expected to see the same pattern (in western blotting), but...
03 April 2012 9,820 13 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...
02 March 2021 9,157 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...
22 February 2021 1,789 3 View
This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...
22 February 2021 338 9 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View
I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
18 February 2021 6,736 4 View
