How long can be kept the freeze dried plant extract, at -20 °C?
Ca we filter the cell culture media containing Pen/Strep?
05 June 2019 4,183 4 View
Does anybody know about ICI journals and their validation?
01 February 2018 431 0 View
Why DNA polymerase can not start DNA synthesis without free 3'-OH, but RNA polymerase can do this? What structural difference(s) have made this possible?
04 May 2016 5,420 14 View
Can anybody answer my question? You have determined the DNA-binding site of a protein by DNA footprinting after labeling one strand. To check your results, you repeat the experiment after labeling...
31 December 2013 3,885 1 View
Does cDNA have a pattern on gel electrophoresis?
04 May 2012 3,939 0 View
I have treated my cells with two different drugs. One of these was disssolved in MeOH and the other in DMSO. In the control samples, we expected to see the same pattern (in western blotting), but...
03 April 2012 9,994 13 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View