Are there commercially available GFP-Expressing HUVECs for angiogenesis assay which have not been infected with lentivirus?

Dear all, I am interested in setting up an Angiogenesis assay on our Essen IncuCyte system but the cells which are recommended are infected with Lentivirus which we can not use due to our labs...

29 November 2020 2,350 1 View

Interaction terms and singularity errors?

Hi, I am currently trying to produce a linear regression model. I have used hierarchial regression stechniques to produce a multivariable model. I have then looked for interaction terms and added...

03 December 2019 4,201 3 View

Best Protocol for Biosynthetic Gene Cluster Heterologous Expression?

Hi, Does anyone have any recommendations on what methodology to use for the direct cloning and heterologous expression of a 69kb NRP gene cluster from Gram-positive bacteria? Thank you!

06 November 2019 3,179 3 View

Designed primers ran on a gel, not working?

Hi, I am looking for advice. I designed my own primers for human TBP, TGFB1 and ITGB6 Gene's. To test my primers I ran a PCR and gel, however, it didn't work. Does anyone have any solutions? I...

20 March 2019 3,950 3 View

Does anyone know what this rectangle on my western blot is?

I have been running western blots to verify the presence of a protein in my co-IP but a strange rectangle keeps showing up on my membrane and blocks the signal when I image it. I reran my western...

16 January 2019 847 4 View

Caco2 cell line passage number and Pgp presence?

What is the passage number of caco2 cell line that has the highest expression of Pgp? I remember that there was a range at which the expression increases but after that passage number it is no...

11 September 2018 7,436 1 View

How do I clear Mycoplasma arginini contamination from my viral RNA sample for NGS sequencing?

Hello, I have extracted my RNA from a virus and I want to sequence using NGS, the RNA sample has Mycoplasma arginini contamination. I would like to eliminate the bacterial contamination before...

06 March 2018 7,380 2 View

Anyone know how to do a data dictionary for a System Dynamic model and develop a user friendly web based tool for public use of the SD model?

What software to use, previous works done in the same area?

28 May 2016 8,155 4 View

Any advice on measuring phenolic content?

08 October 2015 8,010 10 View

Is there a specific T-type calcium channel inhibitor that can be used in vivo?

I want to investigate the behavioural effects of selective inhibition of T-type calcium channels compared to L-type inhibition. I know that there are some anti-epileptic drugs that have been...

21 November 2013 3,819 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View