We have an issue with lateral flow assay where the conjugate isn't fully released initially. The residual conjugate was then releases later after 8 min. potentially interfering with the test line results. What is the possible root cause for conjugate not fully released initially? Thank you in advance for your help.
gradual and steady release of the conjugate is the desired outcome, however, a mixture of conditions can lead to release problems.
First. Check that the conjugation process is not causing clumps or large aggregations. This could be a sign that your conjugate is sticking to the paper surface or you have large chunks that take longer t release
Second. The conjugate pad pre-treatment might need some adjustments, try increasing the concentration of your detergents.
finally, are you using a lot of sugars or a reagents that might harden and might interfere with the flow?
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