Are there commercially available GFP-Expressing HUVECs for angiogenesis assay which have not been infected with lentivirus?

Dear all, I am interested in setting up an Angiogenesis assay on our Essen IncuCyte system but the cells which are recommended are infected with Lentivirus which we can not use due to our labs...

29 November 2020 2,350 1 View

Interaction terms and singularity errors?

Hi, I am currently trying to produce a linear regression model. I have used hierarchial regression stechniques to produce a multivariable model. I have then looked for interaction terms and added...

03 December 2019 4,201 3 View

Best Protocol for Biosynthetic Gene Cluster Heterologous Expression?

Hi, Does anyone have any recommendations on what methodology to use for the direct cloning and heterologous expression of a 69kb NRP gene cluster from Gram-positive bacteria? Thank you!

06 November 2019 3,179 3 View

Designed primers ran on a gel, not working?

Hi, I am looking for advice. I designed my own primers for human TBP, TGFB1 and ITGB6 Gene's. To test my primers I ran a PCR and gel, however, it didn't work. Does anyone have any solutions? I...

20 March 2019 3,950 3 View

Does anyone know what this rectangle on my western blot is?

I have been running western blots to verify the presence of a protein in my co-IP but a strange rectangle keeps showing up on my membrane and blocks the signal when I image it. I reran my western...

16 January 2019 847 4 View

Caco2 cell line passage number and Pgp presence?

What is the passage number of caco2 cell line that has the highest expression of Pgp? I remember that there was a range at which the expression increases but after that passage number it is no...

11 September 2018 7,436 1 View

How do I clear Mycoplasma arginini contamination from my viral RNA sample for NGS sequencing?

Hello, I have extracted my RNA from a virus and I want to sequence using NGS, the RNA sample has Mycoplasma arginini contamination. I would like to eliminate the bacterial contamination before...

06 March 2018 7,380 2 View

C10 cells not growing?

I just started working with an immortalized C10 cell line. The new batch was defrosted by standard methods but is not growing. Often not dying, but not proliferating. Cells have long projections...

11 September 2017 3,317 3 View

Anyone know how to do a data dictionary for a System Dynamic model and develop a user friendly web based tool for public use of the SD model?

What software to use, previous works done in the same area?

28 May 2016 8,155 4 View

Any advice on measuring phenolic content?

08 October 2015 8,010 10 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View