Are there commercially available GFP-Expressing HUVECs for angiogenesis assay which have not been infected with lentivirus?

Dear all, I am interested in setting up an Angiogenesis assay on our Essen IncuCyte system but the cells which are recommended are infected with Lentivirus which we can not use due to our labs...

29 November 2020 2,350 1 View

Interaction terms and singularity errors?

Hi, I am currently trying to produce a linear regression model. I have used hierarchial regression stechniques to produce a multivariable model. I have then looked for interaction terms and added...

03 December 2019 4,201 3 View

Best Protocol for Biosynthetic Gene Cluster Heterologous Expression?

Hi, Does anyone have any recommendations on what methodology to use for the direct cloning and heterologous expression of a 69kb NRP gene cluster from Gram-positive bacteria? Thank you!

06 November 2019 3,179 3 View

Designed primers ran on a gel, not working?

Hi, I am looking for advice. I designed my own primers for human TBP, TGFB1 and ITGB6 Gene's. To test my primers I ran a PCR and gel, however, it didn't work. Does anyone have any solutions? I...

20 March 2019 3,950 3 View

Does anyone know what this rectangle on my western blot is?

I have been running western blots to verify the presence of a protein in my co-IP but a strange rectangle keeps showing up on my membrane and blocks the signal when I image it. I reran my western...

16 January 2019 847 4 View

Caco2 cell line passage number and Pgp presence?

What is the passage number of caco2 cell line that has the highest expression of Pgp? I remember that there was a range at which the expression increases but after that passage number it is no...

11 September 2018 7,436 1 View

How do I clear Mycoplasma arginini contamination from my viral RNA sample for NGS sequencing?

Hello, I have extracted my RNA from a virus and I want to sequence using NGS, the RNA sample has Mycoplasma arginini contamination. I would like to eliminate the bacterial contamination before...

06 March 2018 7,380 2 View

C10 cells not growing?

I just started working with an immortalized C10 cell line. The new batch was defrosted by standard methods but is not growing. Often not dying, but not proliferating. Cells have long projections...

11 September 2017 3,317 3 View

Anyone know how to do a data dictionary for a System Dynamic model and develop a user friendly web based tool for public use of the SD model?

What software to use, previous works done in the same area?

28 May 2016 8,155 4 View

Any advice on measuring phenolic content?

08 October 2015 8,010 10 View

Petrel- well coordinates issue?

Hi, After loading 2D seismic lines in Petrel, I added three exploratory wells ( which supposed to be within the same 2D area) but these wells appears off the interesting area when I visualized...

25 October 2020 8,272 4 View

What are the data needed for an EOR study to run simulation on Eclipse simulator and Petrel ?

I am an Msc Student and chose to study what is the best EOR strategies for Ekofisk oil field by using eclipse simulator and petrel software but im confused about exactly what data and information...

10 April 2020 6,271 3 View

Salt dome modelling in Petrel?

Dear All, Does anyone know how to treat salt domes in the gridding process of Petrel? I have my horizons as regular surfaces and my salt intrusion is triangle mesh but I fail to incorporate the...

16 April 2019 1,898 4 View

Petrel- 2D seismic data loading?

Hi, While loading 2D seismic lines in Petrel, I get warning on "Duplicate traces". If I continue, the displayed section shows like in blocks. I have seen the segy files in Seisviewer and Vista,...

08 February 2019 9,650 4 View

How can I build a 3D velocity model (on a previously constructed 3D structural framework) using only checkshots in Petrel software?

I'm trying to build a 3D velocity model (on a previously constructed 3D structural framework) in Petrel Software using only checkshots. Can anyone help me, please?

17 November 2018 7,138 36 View

Guys, is it possible to export geological model from Petrel to Comsol Multiphysics?

Enhanced oil recovery modeling

09 October 2018 3,694 1 View

Creating a new coordinate system in PETREL?

22 July 2018 7,099 14 View

Creating a new coordinate system in PETREL?

06 July 2018 2,625 15 View

How can i relate a rock physics function to an special facies in Petrel Software?

i have five facies and there are five rock physics functions (each one relates to an special facies), but i don't know how to connect them together!

10 November 2017 3,695 1 View

How to calculate saturation and permeability on petrel?

I have ILD, gamma ray, density and porosity log on PETREL and I wanted to estimate the saturation of oil and water and permeability as well as the oil-water contact. Can anyone give me some ideas...

10 November 2017 5,834 5 View