Hello,
Dose the presence on bug/internal loop in 5-end of miRNA helix with the target mRNA affect the function of mir?
Hi, Am working on MCF-7 breast cancer cell line and I want to do in vivo study , Can MCF-7 produce tutor in vivo without estrogen implants? Also, I have another model where the control group in...
03 April 2018 4,732 3 View
Hello, Do any one know how TCGA database works? any tutorial to show ho to use it and interpret the data? Also, any idea about any database website that shows the expression level of specific...
07 August 2016 3,752 2 View
I have some reading in my MTT assay above 1.5, Is there range for correct reading? What is the plateau or the maximum reading in MTT assay? Thanks
05 June 2016 8,116 0 View
In WB the protein level was lower in the overexpression cell line My antibody is against human ,rat and mouse I did an overexpression of my protine using human cDNA in a normal mouse mammary...
05 June 2016 2,059 4 View
Hello,I am looking for a database bioinformatic tool to show the expression of microRNA in normal and malignant breast tissue, Thanks
04 May 2016 1,580 4 View
Hello,I am looking for primary normal human cell line wit a good price?
02 March 2016 6,520 6 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
18 July 2024 10,010 0 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View