Dear Faris,

I think that the best way for you is to establish a comprehensive collaborative network in your prestigious University with surgeons and pathologists to establish your primary cell lines of interest.

The precise question is in fact: what do you mean by "primary normal human cell lines?".

Best regards

Robert

Hi Faris!

First you have to decide which type of primary cell line do you want ( is it epithelial, endothelial , keratinocytes , melanocytes , ...etc) and then specify, if you choose the epithelial ( which type of the epithelial cells do you want, is it from mammary, bladder , prostate, .... etc).

We used to buy our cell lines from ATCC and they have wide range of primary human cell lines ( Normal ).

The estimated cost depends on the type of cell line , you can find the umbilical vein endothelial for £169 , or the primary dermal fibroblast for £185. On the other hand, the primary mammary epithelial will cost you £659.

kind regards

Nadia

Thanks 

Was really helpful 

Dear Faris,

I totally agree with Nadia! Initially, you have to decide what type of human cell line you want to culture in your laboratory. There are too many types. Then, besides ATCC company, you should also check Lonza, Promo Cell and finally Stem Cell Technologies. You will find many cell lines and also protocols that will help you!

Kind Regards,

Vasileios

I am also looking for oral cancer cell line such as sas or hsc 2 or 3 with good price. Do you know where i can get them.  I ve checked atcc web and couldnt find it

Hi, I do not know what kind of normal cell line you need, but I bought a cryovial of immortalized normal ovarian cell line from Inqapa company which is cost me ~ $2000.