BSA + DHPE suspenssion?

David Rojas Márquez @David-Rojas-Marquez

05 November 2019 0 7K Report

Hi guys!, I'm looking for a protocol to resuspend my DHPE dye in a BSA solution. I want to stain the plasma membrane of Bone Marrow Derived Macrophages with this dye, but the thing it's that you need a organic (chlorophorm) solution, or conjugate it with a protein (BSA). Does anybody know how to perform this type of conjugation?. What BSA concentration should I use?, how long I have to incubate my cells with this suspenssion? Thanks for your time!

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