I just took out a POD-labeled antibody from the frost-free -20oC where I had kept it last summer (so it is a year old now). It has a brownish tinge to it and I can't remember if it was colourless at the time that I stored it. Can anyone tell me if the antibody is still good to use?
Thanks!
HRP in concentrated form has inherent brown color. Generally frost free deep freezer is not recommended for storing immunochemicals. For checking antibody activity you have to try simple immunochemical interaction with immobilized antigen.
I actually just did a series of dilutions of the antibody (1:1 to 1:10,000) and added 1X substrate to it (TMB/H2O2) and I was able to detect a coloured reaction in all tubes, suggesting that my antibody is still fine for use.
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