Can anyone help with gene duplication and phylogenetics?

I have a dataset of approx 150 DNA sequences of a gene with 450 bp length. The dataset contains genes for related species with divergence times of about 26 mya, and gene duplication is widely...

04 May 2014 4,277 8 View

How could the selection intensity among loci be measured?

I've been genotyping genes that have been under duplication, so I want to know if there is a method to measure selection among those loci under duplication to find differences among them.

31 December 2013 4,623 5 View

Homology modelling or in silico mutation.

I want to get the structure of different proteins. But their similarity is about 95%. There are some mutations distributed in a region of the protein. Which is the best way to obtain a good model,...

06 July 2013 1,563 9 View

Does anyone know any software or servers to calculate desolvation free energy between two proteins?

I am trying to get thermodynamic measures for a protein protein docking, but I want to take as input my own generated pdb files. I have tried Fastcontact server and it works in several cases but...

01 February 2013 8,873 1 View

Does anyone know a way to obtain the labels of distances between atoms in plain text with pymol?

I am tryinng to measure different distances in different protein protein dockings i have made. I want to export the distances to plain text to make statistical analysis.

10 November 2012 2,230 1 View

How can I predict possible miRNAs in a genome, having as a template the 3' UTR of a transcript?

I have 3' UTRs from different transcripts and I want to predict possible miRNAs regulating that transcripts in a genome.

10 November 2012 3,165 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View